Tryst with Destiny: On this day, August 15 in 1947, India gained independence as a new nation.

Tryst with Destiny: On this day, August 15 in 1947, India gained independence as a new nation. The midnight speech of Jawaharlal Nehru, the first Prime Minister of India (pictured on left, with Mahatma Gandhi)  to the Indian Constitutent Assembly began, “Long years ago we made a tryst with destiny, and now the time comes when we shall redeem our pledge, not wholly or in full measure, but very substantially. At the stroke of the midnight hour, when the world sleeps, India will awake to life and freedom. A moment comes, which comes but rarely in history, when we step out from the old to the new, when an age ends, and when the soul of a nation, long suppressed, finds utterance.” The speech is considered a masterpiece of oration and marked the triumph of the nation’s 100-year largely non-violent struggle for independence. 

Happy Birthday, India!

Nehru (14 November 1889 – 27 May 1964) was a noted pacifist, poet and prolific author. A lawyer by profession, he was inspired to join the freedom fighters by Gandhi. It was Nehru who ensured secularism by focusing on the plurality of the Indian people. His daughter, Indira Gandhi, also became Prime Minister.

Tryst with Destiny Speech: http://en.wikipedia.org/wiki/Tryst_with_destiny

Independence History: http://en.wikipedia.org/wiki/India%27s_independence_movement

Nehru Quotes: http://www.spiritquotes.com/quotes/jawaharlal-nehru-quotes-jawaharlal-nehru-sayings.htm

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Meanwhile, at the fly Olympics…      Photo credit: Magnus Muhr

Meanwhile, at the fly Olympics…      Photo credit: Magnus Muhr

#london2012

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Gender Bender: How do you tell a man from a woman?

Gender Bender: How do you tell a man from a woman? The International Olympic Committee has decided to use testosterone levels to decide who can compete as a woman. But it’s not that simple: testosterone levels of elite athletes, both male and female, spread out over a range and overlap as seen in this scatter plot: http://goo.gl/YIKFQ

• Besides, there are no studies showing that athletes with higher testosterone compete better in sports. Neither is there evidence that response to hormone is the same between individuals. An extreme case is complete androgen insensitivity syndrome. These individuals are chromosomally XY, the normal makeup for men, but their bodies don’t respond to testosterone. So they develop female genitals, but have testes, not ovaries.  South African powerhouse runner Caster Semenya is thought to be one such person. She was banned from competing and then, mysteriously, brought back this year (http://goo.gl/cGyIb). 

• Half a century ago, the IOC subjected women athletes to “nude parades” before a panel of judges. After realizing that outward appearance can be confounding (as a result of adrenal gland abnormality, for example), they tested for Barr bodies, characteristic of XX chromosomes. But females can have a single X chromosome. This was followed by testing for the SRY gene thought to determine male gender (See my Men! Why U So SRY? post http://goo.gl/VPF0J). But the Atlanta Olympics revealed 8 female athletes who carried this gene, all of whom were eventually allowed to complete.

• Naturally high testosterone in women is a genetic trait, no different from having more efficient muscles or acromegaly (tallness). So why should some athletes be penalized for this particular trait? Clearly, the goal is to prevent unfair advantage in sports. But it’s complicated….

Image: http://www.nataliedee.com/archives/2010/Apr/

#london2012 #scienceeveryday  

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Cellular Google+ Circles: Don’t You ❤ This?

Cellular Google+ Circles: Don’t You ❤ This? All part of the daily dance show put on by our cells. This time lapse movie shows a cell that was activated by a growth factor. In a coordinated synchrony of motion, the underlying cytoskeleton rearranges to form circular dorsal ruffles that coalesce in a happy coincidence to form a heart.

Image: I ♡Kevin Staff for donating his time and expertise to support ScienceSunday here on Google+. If you don’t have him in your circles, you are missing out on beautiful imagery, poetry and animations. Thanks Kevin!

Source: http://www.cellimagelibrary.org/images/7762

Video taken by Hongying (Hoy) Shen (Yale University; Dr. Pietro De Camilli Lab).

#sciencesunday ScienceSunday 

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A Double Dose of Dosas: The Southern Indian Crepe.

A Double Dose of Dosas: The Southern Indian Crepe. The gum-chewing, bored looking US customs officer gave me an expert once-over as I dragged my world weary rag tag family towards the airport exit. “Any mangoes, miss?” I have a theory that men in uniform have been trained to call all women Miss, even if it is obvious that we are biologically and socially old enough to qualify as their mother.

He turned his attention to the oddly sized package on our luggage cart, “What’s that?”

I straightened my aching, tote bag burdened shoulders with pride. “That is a table top granite wet grain grinder”. Pausing only to note his mild interest, I launched forth saying more or less the following (possibly, more): It has two granite cones that rotate within a motorized, spinning stainless steel chamber with an attached paddle and stone base.  It is used for grinding lentils and rice to a fine, fluffy batter that is fermented to make dosas. An ordinary blender with a steel blade is marginally adequate but a grinder is best for the authentic silky feel to the crepe.

Read on, with recipes: https://madamescientist.wordpress.com/2012/07/29/a-double-dose-of-dosas-two-southern-indian-crepes/

Masala dosas: Recently voted the number one food from around the world to try before you die. Not to hasten you to an early grave, but you must experience the dosa.

Cabbage Adai: Should the microbial reactions in the fermentation prove too off putting or enzymatically challenging for some, I offer you a non-fermented variation with less prep time. This is a protein and lentil rich, crunchy dosa known as Cabbage Adai.

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A Double Dose of Dosas: Two Southern Indian Crepes

The gum-chewing, bored looking US customs officer gave me an expert once-over as I dragged my world weary rag tag family towards the airport exit. “Any mangoes, miss?

I have a theory that men in uniform have been trained to call all women Miss, even if it is obvious that we are biologically and socially old enough to qualify as their mother.

“No, haha!”, I responded, thawing on cue and smiling confidentially. He could have charmed my ATM password out of me at this point, had I known it myself (which I don’t).

How about meat products?” My eyebrows drew together in disapproval. “Of course, not”.  Was it not obvious from our ascetically, if not aesthetically, scrawny slender musculature that we had been weaned on protein poor vegetarian food since birth?

Hastily, aware of his slip, he turned his attention to the oddly sized package on our luggage cart, “What’s that?

I straightened my aching, tote bag burdened shoulders with pride. “That is a table top granite wet grain grinder”. Pausing only to note his mild interest, I launched forth saying more or less the following (possibly, more): It has two granite cones that rotate within a motorized, spinning stainless steel chamber with an attached paddle and stone base.  It is used for grinding lentils and rice to a fine, fluffy batter that is fermented to make dosas. An ordinary blender with a steel blade is marginally adequate but a grinder is best for the authentic silky feel to the crepe.

Wet Grain Grinder aka Kitchen Conversation Piece.

Wet Grain Grinder aka Kitchen Conversation Piece.

Inside view of grinder.

Inside view of grinder.

Somewhere in the midst of this monologue, I caught the warning glint in my husband’s eye. You know, the one that is reserved for the rare times my professorial pontifications get the better of me. Right, moving along. It’s not like I was using a PowerPoint presentation, I muttered to myself indignantly. Actually, I did do just that at Airport Security in Tel Aviv. But that’s another tale.

This one goes out to all the Customs Officers, Security Personnel and Police who shamelessly flatter by calling me Miss.

Masala dosas: Recently voted the number one food from around the world to try before you die. Not to hasten you to an early grave, but you must experience the dosa. There are multiple steps and prior planning involved: If you have not made these before, it helps, as we say to the summer students in my lab, to pay attention. (I didn’t take as many photographs as I should have, so I’ll update with more images later).

  1. Make the Batter
  • Separately soak rice and urad dal  (Vigna mungo) under an inch of water for 6-8 hours or overnight. Confusingly, this lentil is black when whole, and white when halved and hulled.

If you like your dosas soft, use a 3:1 ratio. If you like them crisp, increase the proportion of rice to 4:1. I used 3.5 cups of rice and 1 cup of urad dal.

  • Grind the softened lentils for 20 minutes in the granite grinder with enough water to make a smooth batter. If you don’t have this useless conversation piece, go ahead and use an ordinary blender.

The batter is perfect when a fluffy spoonful floats when dropped into water!

Lentil batter so light and fluffy that it floats.

Lentil batter so light and fluffy that it floats.

  • Scoop it out and transfer to a large pot with enough room for rising. Do the same with the softened rice: this is going to have a more gritty texture.

Grinding the rice.

Grinding the rice.

  • Mix in with the lentil batter along with a teaspoon of salt.

Dosa batter, just ground.

Dosa batter, just ground.

  • Cover, and place in a warm spot to ferment. My remodeled kitchen has a warming drawer that can be set to 85F, perfect for culturing yogurt, raising dough or fermenting dosa batter. I’ve also left the lamp on in my oven to provide mild warmth.

Let rise in a warm spot.

Let rise in a warm spot.

After an overnight incubation, the batter should be full of bubbles. Don’t let it go too long, or the batter gets excessively sour (refrigerate, if you are not ready to use it).

Yeast Alive! Fermented batter.

Yeast Alive! Fermented batter.

  1. Make the Chutney

To make a chutney, you will need a bunch of coriander (cilantro), a cup of grated coconut (I buy mine pre-grated from the frozen section of an Indian grocery store), a teaspoon of tamarind pulp (from soaking dry tamarind pods, or as a concentrate), a few green chilies, and spices for tempering.

  • Grind all the ingredients (other than tempering spices) together in a blender, using judicious aliquots of water, titrating it to the right chutney consistency. Season with salt and remove to a bowl.

Coriander bunch, green chilies and coconut.

Coriander bunch, green chilies and coconut.

Tamarind concentrate.

Tamarind concentrate.

  • Temper the chutney with a teaspoon each of urad dal and mustard seeds, plus 1-2 dry red chilies. Heat together in a small pan until the mustard seeds turn gray and pop.

You can flavor with a pinch of asafoetida if you are a purist. (Other chutney versions use garlic. I don’t out of deference to my late grandmother whose disapproval would time-travel through the etheric past to haunt me). Toss atop the chutney for a satisfying sizzle. Your kitchen will smell delightfully of fresh herb and roasted nuts, unless you used too much asafoetida in which case it will not.

Coriander chutney

Coriander chutney

  1. Make the Potato Filling
  • Boil a few potatoes, peel and roughly cube. Chop a small onion. You can chop a few green chilies, or leave them whole.
  • Begin with the same tempering as with the chutney above. This flavors the oil and provides a nutty crunch in contrast to the soft and creamy potato filling.
  • Lightly saute the onions. Add the cubed potatoes and chilies. Season with salt, and turmeric for that golden color.

Potato stuffing for dosa

Potato stuffing for dosa

  • Add a few tablespoons of water and mash the potatoes with the back of your spoon to make the mixture soft and spreadable. Finally, squeeze on some lemon juice and garnish with chopped cilantro. You can add cashew pieces too.

To Make Dosas:

Heat a nonstick or seasoned cast iron griddle. Set aside some vegetable oil. I add some toasted sesame oil for a more aromatic mix.

  • Pour a ladleful of batter in the center. Using the back of the ladle and gentle pressure, quickly make an outwardly spiraling circle. The alternating thin/thick texture of the dosa conveys both soft and crispy textures in every delicious and contradictory mouthful. Sprinkle about a teaspoon of oil, cover with a lid. After a couple minutes, test the edges to see if it releases easily. Fermented dosas cook quickly.

Dosa batter on a griddle.

Dosa batter on a griddle.

  • Spread first chutney and then potato filling over half the dosa surface and then flip the other half over. Other dosa conformations include triangle and roll. Serve hot and golden! Top with unsalted butter or ghee for a rich flavor. (I ran out of gun powder, alias chutney pudi. That intriguing condiment awaits my upcoming trip to India).

Spread with chutney and potato stuffing, then fold over.

Spread with chutney and potato stuffing, then fold over.

Take Two: Adai (non fermented dosa)

Should the microbial reactions in the fermentation prove too off putting or enzymatically challenging for some, I offer you a non-fermented variation with less prep time. This is a protein and lentil rich, crunchy dosa known as Cabbage Adai.

You will need rice, and four types of lentils: Tuvar dal, Channa dal, Urad dal and Moong dal.

From L to R: Toovar, Channa, Urad and Moong dals.

From L to R: Toovar, Channa, Urad and Moong dals.

  • For one measure each of the four dals, use 1.5 measures of rice. I used a quarter cup measure.

Mix lentils and rice in the ratio 1:1:1:1:1.5

Mix lentils and rice in the ratio 1:1:1:1:1.5

  • Soak the rice and dals with a few pepper corns and dry red chillies for a couple of hours.

Presoaked rice and lentils

Presoaked rice and lentils

  • Grind it all together. An ordinary high-speed blender is fine. Leave some bits for texture.

Blend together rice and lentils

Blend together rice and lentils

Batter for Adai

Batter for Adai

  • Chop about a third of a small cabbage finely, and add it to the batter with salt to taste.

Slice cabbage lengthwise

Slice cabbage lengthwise

Then crosswise...

Then crosswise…

  • Make the dosas as before.

Spread out the batter.

Spread out the batter.

  • This time, you need to flip them since these dosas are thicker and need a couple more minutes to cook.

Flip it once to cook the other side

Flip it once to cook the other side

Adai closeup.

Adai closeup.

  • Serve with chutney.

Cabbage Adai with Coriander Chutney.

Cabbage Adai with Coriander Chutney.

There are endless variations on the dosa. You can use different flours (wheat, semolina), different toppings (fresh tomatoes, cilantro and onions) and make them without the tedium of presoaking and grinding. Remember, the clock is ticking so try them any which way!

Posted in FOOD, Humor, Indian food, Spices | Tagged , , , , , | 15 Comments

The Importance of Stupidity in Research: Today, on that other social network, my grad student Brandie Cross tagged…

The Importance of Stupidity in Research: Today, on that other social network, my grad student Brandie Cross tagged me on this GIF  with the title, “My PI when I try to explain why my experiment failed”. I laughed. I was reminded of a wonderful essay by microbiologist Martin Schwartz that made the case for productive stupidity in science. It’s great to feel smart, but it’s more useful to feel stupid. If you don’t feel stupid, are you really pushing the envelope, stretching for that tantalizing little nugget of knowledge seemingly just beyond your reach?

• Albert Einstein said, “If we knew what it was we were doing, it would not be called research, would it?”, which has been loosely parsed as the first law of scientific research: E = Herp Derp^2. The beauty of science is that it is perfectly okay to bumble along , mostly getting things wrong, but being insanely elated when we learn something. The more comfortable we become with being stupid, the deeper we will wade into the unknown and the more likely we are to make big discoveries. Hey, it’s not that bad being scorned by the divine Meryl Streep 🙂

REF: http://jcs.biologists.org/content/121/11/1771.full

GIF: “My PI when I try to explain why my experiment failed”, http://goo.gl/96ub1

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Science is a way of thinking much more than it is a body of knowledge.

Science is a way of thinking much more than it is a body of knowledge.

Carl Sagan

Originally shared by Science on Google+

We’re making some changes to the Science on Google+: A Public Database page. To kick off these changes, we are pleased to announce that three new Scientists/Google+ enthusiasts have agreed to help curate the page and database along with Chris Robinson and Rajini Rao. 

Jon Hiller, curator of Applied and Mathematical Sciences

Scott Lewis, curator of Natural Sciences

Erin Kane, curator of Social Sciences and  Communication

We are also excited about a few additional changes that are coming in the near future. Stay tuned! As always, feel free to:

• Browse the database: http://goo.gl/Yz8KR

• Add discipline specific shared circles: http://goo.gl/sSzcl

• Add your profile/page to the database: http://goo.gl/yEg7M

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Would you give up sex for eternal life?

Would you give up sex for eternal life? This little invertebrate, a bdelloid rotifer, has lived a celibate life for ~80 million years. The males have disappeared and the females reproduce by parthenogenesis. A drawback to this convenient scheme is that our DNA is usually repaired during meiosis, when we form gametes or germ cells.

• Scientists bombarded these little creatures with gamma rays that would typically shatter DNA into little bits. To their astonishment, the rotifers kept reproducing even at levels of radiation five times more than other animals can endure. Their secret lies in genetic redundancy: their genomes have duplicated, so that each gene is in 4 copies. When one is damaged, the others serve as template to copy off a new version (gene conversion).

• How did this resistance to radiation damage evolve? These animals live in fresh water pools that can dry up at any time. The rotifers can go dormant for weeks to years, springing back to life with water. Dessication has the same effect on DNA as radiation so the rotifers must have evolved to survive in their ephemeral habitats. “There could be some benefit to millions of years without sex after all”, says Dr. Alan Tunnacliffe, University of Cambridge 🙂

Live image of Philodina roseola , details at http://www.cellimagelibrary.org/images/41670

Refs: (1) Gladyshev, E., and M. Meselson. 2008. Extreme Resistance of Bdelloid Rotifers to Ionizing Radiation. Proc. Natl. Acad. Sci. 105 (13): 5139-5144.

(2) Mark Welch, D.B., J.L. Mark Welch and M. Meselson. 2008. Evidence for degenerate tetraploidy in bdelloid rotifers. Proc. Natl. Acad. Sci. 105 (13): 5145-5149.

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Asking for Help: If you have experience with Bi-Polar Disorder or know of someone who can help, could you please…

Asking for Help: If you have experience with Bi-Polar Disorder or know of someone who can help, could you please contact our friend Greg? Please get the word out. Thanks so much.

Originally shared by Gregory Esau

G+ and Glia Community

We Need Your Help

This is a challenging post to write. My brother, Brad Esau , suffers immensely from Bi-Polar disorder. 

The ‘traditional’ pharmaceutical approach is slowly killing him. 

We need to find a different approach, and a different approach very soon.

Here is what I am asking and proposing.  Brad needs a support community, a circle of professionals and fellow BP suffers that he can connect to here in G+. I know that there is a vast wealth of resources here, and much better alternatives than the drugs he is now on. 

If any of you could help us connect to the kind of professionals, or those who have deep experience, or anybody that can understand, or support, or connect to Brad in a meaningful way, this would be fantastic. 

Brad, while not desperate, is in dire need of radical change. He has gone off much of his medication, which means he will be hospitalized within a week. He is doing this because he cannot continue living as he has, like a walking zombie full of drugs.  

He will tell his story here in time, but we need to find the right circle of people he can share that story with. 

I hope you can help us. Thank you so much in advance

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